MOLECULAR INSIGHT :
We have developed an original Treg Molecular Signature (TregSign kit) which allows, in a single assay, a complete transcriptomic study of Tregs.
The signature allows us to determine the impact of a molecule on Tregs and their microenvironment
The signature is based on the expression of around 100 genes associated with both natural Treg and induced Tregs. 8 clusters were established to better assess Treg biology :
Phenotype Suppressive function Expansion Induction Adhesion Activation state Migration Infiltration
Potential clinical applications: Evaluation of compounds on Tregs/PBMCs Patient immuno-monitoring – Strong diagnostic and prognostic value – Improvement of patient stratification according to Treg profile for better treatment choice
CELLULAR INSIGHT :
PBMC/Treg Supression Assay
We offer a wide range of methods to comprehensively assess the efficacy and safety of your compound of interest on Treg Biology. Immunomonitoring and Immunophenotyping of Tregs by :
Suppressive Function Assays
Recruitment Analysis
Secretion Profile
Cell Death & Viability Assay
Tumour Infiltration Analysis
ANIMAL INSIGHT :
In vivo assessment of a compound efficacy and safety can be carried out on a human cancer model designed for you (Syngeneic, PDX-Like models…).
PDX-Like model: Humanized SCID mice allow a complete monitoring of disease advancement, organ infiltration, toxicity etc.
REFERENCES
Bibliography of Immune insighT and its founding members:
IST: Immune insighT
Humanized PDX-Like model POC
Clinical trial immunomonitoring
TregSign Kit POC
Review